The Aneufast™ QF-PCR Kit is a molecular diagnostic assay designed for rapid detection of chromosomes 13, 18, 21, X and Y aneuploidies by Quantitative Fluorescent PCR (QF-PCR) with 100% sensitivity and specificity for non-mosaic trisomies. The kit contains a total of 36 markers in six multiplex reactions that amplify selected Short Tandem Repeats (STRs) and the gender determining sequences Amelogenin-SRY. Two multiplex QF-PCR amplifications of 21 markers (S1 and S2) are designed to be analysed in a single electrophoresis to increase sample throughput. The inclusion of 4 chromosome specific extra markers sets allows definitive results in 100% of cases.
Aneufast™ has been developed for the amplification of DNA extracted from fresh prenatal samples such as Amniotic Fluids, Chorionic Villus Samples (CVS) or fetal blood. It can also be used to analyze neonatal and adult blood or tissue samples.