Chromosomal abnormalities, alterations and aberrations are at the root of many inherited diseases and traits. Chromosomal abnormalities often give rise to birth defects and congenital conditions that may develop during an individual’s lifetime. Numerical chromosome abnormalities (aneuploidies) can occur with either the autosomes or the sex chromosomes. Monosomy is the absence of a chromosomal region or entire chromosome, resulting in only one copy of the genetic material, while trisomy is the presence of an extra chromosomal region or entire chromosome, resulting in three copies of the genetic material.

Product Overview

The Aneufast™ QF-PCR Kit is a molecular diagnostic assay designed for rapid detection of chromosomes 13, 18, 21, X and Y aneuploidies by Quantitative Fluorescent PCR (QF-PCR) with 100% sensitivity and specificity for non-mosaic trisomies. The kit contains a total of 36 markers in six multiplex reactions that amplify selected Short Tandem Repeats (STRs) and the gender determining sequences Amelogenin-SRY. Two multiplex QF-PCR amplifications of 21 markers (S1 and S2) are designed to be analysed in a single electrophoresis to increase sample throughput. The inclusion of 4 chromosome specific extra markers sets allows definitive results in 100% of cases.

Aneufast™ has been developed for the amplification of DNA extracted from fresh prenatal samples such as Amniotic Fluids, Chorionic Villus Samples (CVS) or fetal blood. It can also be used to analyze neonatal and adult blood or tissue samples.

Main Features
  • Sample to result in less than 3 hours.
  • Twenty one (21) highly informative genetic markers amplified in two mixes in order to reduce the risk of sample mishandling.
  • Extra Markers on Chromosomes 13, 18, 21, X and Y included 
in the box .
  • Five Dye-Labeling allows simultaneous analysis of several loci.
  • Only product on the market with 4 pseudoautosomal markers for XY aneuploidy and mosaic cases.
  • Accurate and reproducible results are obtained at input DNA amounts of 1 to 10 ng.
  • Optimized to work on all Applied Biosystems genetic analyzers
  • Manufactured to ISO 9001:2008 and 13845:2003
Order Information
Cat. No Description
mlg.anf.100 100 tests
mlg.anf.50 50 tests
mlg.anf.25 25 tests
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DNA Extraction

15 min.

QF-PCR Amplification

90 min.

Electrophoresis - Analysis

50 min.