Microdeletions of the Y chromosome are the second most frequent genetic cause of spermatogenetic failure in infertile men after the Klinefelter syndrome (47, XXY). The molecular diagnosis of Y chromosomal microdeletions is routinely performed in the workup of male infertility in men with azoospermia or severe oligozoospermia.

The portion of the male-specific region of the Y chromosome (MSY), comprising 95% of the Y chromosome and flanked by pseudoautosomal regions which is affected by deletions, has been classically subdivided into four regions called AZFa, AZFb, AZFc and proximal AZFc (AZFd), respectively.

Product Overview

ydel1This system consists of 14 primer pairs that are homologous to previously identified and mapped sequence-tagged sites (STSs). Y chromosome deletions in the regions that are amplified by these primer sets have been associated with male infertility. This system uses a five-dye fluorescent system for automated DNA fragment analysis, which allows multiplex amplification and electrophoresis of over 14 STSs which include AMXY marker (Chr.X: 104bp, Chr.Y: 109bp; Xp22.1, Yp11.2) simultaneously. The kit is intended for use on Applied Biosystems ABI PRISM® genetic analyzers.


Main Features
  • Contains all necessary reagents for the amplification of human genomic DNA
  • Relative quantification between Chr.X and Chr.Y (AMXY marker) allows the pre-assessment of possible Klinefelter syndrome
  • A human male DNA used as external control to check DNA integrity and PCR inhibitors as well as performance of reagents in the kit
  • Precise and quick detection for all STSs in the same plot screen
  • Coverage of the maximum number of possible Y chromosome microdeletions
  • Contains all 6 markers determined by European Molecular Genetics Quality Network guidelines
  • SRY is a control marker for the testis determining factor on the short arm of the  chromosome and allows XX males arising from Y to X translocations to be detected.
Order Information
Cat. No Description
206100 100 tests
206050 50 tests
206025 25 tests
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DNA Extraction

20 min.

QF-PCR Amplification

120 min.

Electrophoresis - Analysis

30 min.